Abstract Background Rheumatoid arthritis is an autoimmune inflammatory disease that often leads patients to muscle impairment and physical disability. This study aimed to evaluate changes in the activity of proteasome system in skeletal muscles of mice with collagen-induced arthritis (CIA) and treated with etanercept or methotrexate. Methods Male DBA1/J mice were divided into four groups (n = 8 each): CIA-Vehicle (treated with saline), CIA-ETN (treated with etanercept, 5.5 mg/kg), CIA-MTX (treated with methotrexate, 35 mg/kg) and CO (healthy control group). Mice were treated two times a week for 6 weeks. Clinical score and hind paw edema were measured. Muscles were weighted after euthanasia and used to quantify proteasome activity, gene (MuRF-1, PMSα4, PSMβ5, PMSβ6, PSMβ7, PSMβ8, PSMβ9, and PSMβ10), and protein (PSMβ1, PSMβ5, PSMβ1i, PSMβ5i) expression of proteasome subunits. Results Both treatments slowed disease development, but only CIA-ETN maintained muscle weight compared to CIA-MTX and CIA-Vehicle groups. Etanercept treatment showed caspase-like activity of 26S proteasome similar to CO group, while CIA-Vehicle and CIA-MTX had higher activity compared to CO group (p: 0.0057). MuRF-1 mRNA expression was decreased after etanercept administration compared to CIA-Vehicle and CO groups (p: 0.002, p: 0.007, respectively). PSMβ8 and PSMβ9 mRNA levels were increased in CIA-Vehicle and CIA-MTX compared to CO group, while CIA-ETN presented no difference from CO. PMSβ6 mRNA expression was higher in CIA-Vehicle and CIA-MTX groups than in CO group. Protein levels of the PSMβ5 subunit were increased in CO group compared to CIA-Vehicle; after both etanercept and methotrexate treatments, PSMβ5 expression was higher than in CIA-Vehicle group and did not differ from CO group expression (p: 0.0025, p: 0.001, respectively). The inflammation-induced subunit β1 (LMP2) was enhanced after methotrexate treatment compared to CO group (p: 0.043). Conclusions The results of CIA-Vehicle show that arthritis increases muscle proteasome activation by enhanced caspase-like activity of 26S proteasome and increased PSMβ8 and PSMβ9 mRNA levels. Etanercept treatment was able to maintain the muscle weight and to modulate proteasome so that its activity and gene expression were compared to CO after TNF inhibition. The protein expression of inflammation-induced proteasome subunit was increased in muscle of CIA-MTX group but not following etanercept treatment. Thus, anti-TNF treatment may be an interesting approach to attenuate the arthritis-related muscle wasting.

ABSTRACT A trial was carried out to evaluate the effects of different levels of microalgae Schizochytrium sp. on performance, yolk lipid profile, and egg quality of Japanese quail. A flock of 210 quail was distributed in a completely randomized design, with five treatments (0, 10, 20, 30, and 40 g of Schizochytrium sp./kg of feed) and six replications with seven birds per cage. Performance and egg quality were not affected, except for a quadratic effect on yolk color, which reached the maximum value with the inclusion of 40 g of Schizochytrium sp./kg. There was linear reduction in the content of saturated fatty acids and a linear increase of polyunsaturated:saturated and polyunsaturated:monounsaturated ratios and n-6. The content of n-3 showed a minimum value with the inclusion of 6.5 g of Schizochytrium sp./kg, and the n-6:n-3 ratio was maximized with the addition of 10.5 g of microalgae/kg. As for the sensory attributes color, aroma, and overall impression, there was linear increase with the addition of increasing levels of microalgae. The inclusion of up to 40 g of microalgae Schizochytrium sp./kg in the diet of Japanese quail did not present changes in the performance nor in the egg quality but accentuated the yolk color, promoted the fortification of n-3 in the eggs, and still provided excellent sensorial acceptance. The egg fortification can add value to the product, increasing the producer remuneration and improving the nutritional quality of the diet for humans.

To increase yield, breeding programs must search for innovative and efficient methodologies for the development and selection of superior genotypes. Therefore, this study aimed to compare the single- and multiple-trait best linear unbiased prediction (BLUP) in genetic selection of parents and hybrids of grain sorghum. For this, an experiment conducted in alpha-lattice design with two replications was used. Flowering time (FT), plant height (PH), and grain yield (GY) were evaluated in 502 grain sorghum hybrids obtained by the cross of ten restorer lines and 54 male-sterile lines. Variance components were estimated via restricted maximum likelihood (REML). Significant effects of restorer lines, of male-sterile lines, and of specific combining ability were detected by the likelihood ratio test (LRT). The estimates of variance components, genetic parameters, and correlations were similar when obtained via single- and multiple trait BLUP. Considering hybrids, the multiple-trait BLUP resulted in slightly higher predicted selection gain for the three evaluated traits and, therefore, can be efficiently applied in the genetic selection of grain sorghum.

Para incrementar el rendimiento, los programas de mejoramiento deben buscar metodologías innovadoras y eficientes para el desarrollo y la selección de genotipos superiores. De esta forma, este estudio tuvo como objetivo comparar la mejor predicción lineal no viciada (BLUP) mono y múltiple característica, en la selección genética de parentales e híbridos de sorgo granelero. Para esto, fue usado un experimento con diseño alfa latice con dos repeticiones. Se evaluó el tiempo de floración (FT), la altura de planta (AP) y el rendimiento de granos (GY) en 502 híbridos de sorgo, obtenidos por el cruzamiento entre diez líneas restauradoras y 54 líneas macho estériles. Los componentes de varianza se estimaron mediante el método de máxima verosimilitud restringida (REML). Observamos que varias veces el BLUP múltiple característica da como resultado estimaciones de correlación genética más precisas. En este trabajo, se encontraron mayores correlaciones entre FT y GY a través de BLUP múltiple característica. Estos resultados llevaron a mayores ganancias genéticas para la selección de híbridos en comparación con BLUP mono característica.

RESUMO O presente estudo investigou a influência de plateia sobre o desempenho sensoriomotor na aprendizagem do arremesso de dardo de salão. O experimento foi conduzido em dois dias distintos, cada um com uma condição específica: 1) com plateia (10 tentativas); 2) sem plateia (10 tentativas). Os 18 universitários de menor e maior desempenho foram divididos em dois grupos: prejudicado (GP) e beneficiado (GB). Nas análises de desempenho e escores de ansiedade-estado (IDATE), a ANOVA two way e pairwise identificaram interação entre grupo e condição, sendo GP com pior desempenho e maior IDATE e GB com melhor desempenho e sem alteração no IDATE na condição com plateia. Já na frequência cardíaca, não houve interação, porém efeito principal para o fator condição para ambos os grupos. Pode-se inferir que, a plateia influencia no desempenho sensoriomotor podendo ocorrer piora ou melhora a depender de características individuais.

ABSTRACT The present study investigated the influence of audience on the sensorymotor performance in learning the hall of javelin throwing. The experiment was conducted on two separate days, each with a specific condition: 1) with audience (10 attempts); 2) no audience (10 attempts). The 18 students of smaller and higher performance were divided into two groups: disadvantaged (DG) and benefited (BG). The performance and state-anxiety scores (IDATE), the two-way ANOVA and pairwise identified interaction between group and condition, and GP worst performing and most IDATE and GB with better performance and no change in IDATE provided with the audience. In the heart rate, there was no interaction but the main effect for the condition factor for both groups. It can be inferred that the audience influences the sensorymotor performance may cause worsening or improving depend on individual characteristics.

RESUMO JUSTIFICATIVA E OBJETIVOS: A cefaleia é um dos principais sintomas referidos por pacientes com aneurisma cerebral. Como outras dores são, frequentemente, ignoradas pela equipe multiprofissional. Apesar de importante, a avaliação da cefaleia no pré-operatório de pinçamento de aneurisma cerebral é um desafio para a equipe de saúde. O objetivo deste estudo foi avaliar a cefaleia pré-operatória em pacientes submetidos a pinçamento de aneurisma não roto. MÉTODOS: Estudo descritivo, exploratório, de abordagem quantitativa, realizado entre setembro de 2014 e maio de 2015 no setor de neurocirurgia e na unidade de terapia intensiva de um hospital de médio porte. A casuística foi constituída por 28 pacientes no pré-operatório de craniotomia eletiva para o tratamento de aneurisma cerebral não roto. Utilizaram-se os testes de Mann-Whitney, Kruskal-Wallis e Exato de Fisher e adotou-se nível de significância de 5%. RESULTADOS: Demonstrou-se que 78,6% dos pacientes eram do gênero feminino com média de idade de 46,7 anos. A cefaleia pulsátil de localização frontal e intensidade moderada foi a dor mais prevalente. Náuseas e vômitos também estiveram presentes, assim como a dor no globo ocular. A dor piorou com o esforço físico e balanceio da cabeça. Houve diferença estatisticamente significativa para as variáveis: recorrência da dor e dor em globo ocular. CONCLUSÃO: Este estudo esclarece os principais itens a serem investigados na avaliação da cefaleia do paciente com aneurisma cerebral e suas características a fim de facilitar o diagnóstico precoce e a melhora antecipada na recuperação neurológica.

ABSTRACT BACKGROUND AND OBJECTIVES: Headache is a major symptom referred by patients with brain aneurysm. As other types of pain, it is often ignored by the multiprofessional team. Although important, headache evaluation in the preoperative period of brain aneurysm clamping is a challenge for the health team. This study aimed at evaluating preoperative headache of patients submitted to unrupted aneurysm clamping. METHODS: This is a descriptive, exploratory and quantitative study carried out between September 2014 and May 2015 in the neurosurgery sector and the intensive care unit of a medium-sized hospital. Sample was made up of 28 patients in the preoperative period of elective craniotomy to treat unrupted brain aneurysm. Mann-Whitney, Kruskal-Wallis and Fisher Exact tests were used with significance level of 5%. RESULTS: It was observed that 78.6% of patients were females with mean age of 46.7 years. Moderate pulsing and frontal headache was the most prevalent pain. Nausea and vomiting were also present, as well as pain in the eye. Pain has worsened with physical effort and shaking the head. There has been statistically significant difference for the variables pain recurrence and pain in the eye. CONCLUSION: This article clarifies major items to be investigated to evaluate headache of brain aneurysm patients and its characteristics to help early diagnosis and better neurologic recovery.

Neste trabalho foi realizada a triagem das hemoglobinas S e C e o estudo do perfil social de quatro comunidades quilombolas. O estudo foi desenvolvido nas comunidades quilombolas de Malhadinha, Córrego Fundo, Curralinho do Pontal e Manoel João, município de Brejinho de Nazaré (TO). O perfil das hemoglobinas foi obtido com a triagem de 167 amostras de sangue, utilizando o teste de eletroforese em pH alcalino (pH 8,6). Para confirmar o diagnóstico, as amostras alteradas foram submetidas à eletroforese em pH ácido (pH 6,2). Foram identificadas 11 amostras (6,6%) com hemoglobinas variantes: oito (4,8%) com traço falciforme e três com hemoglobina C (1,8%). Os dados sociais foram obtidos através de entrevista realizada com representantes de 48 famílias, levantando informações sobre a renda familiar, idade, sexo e atividade ocupacional de cada indivíduo. O estudo mostrou que essas comunidades são formadas predominantemente por adultos e idosos, com renda mensal maior que um salário mínimo, para 50% das famílias entrevistadas. Apesar do percentual das hemoglobinas S e C encontradas nas comunidades estarem dentro do observado para várias regiões do Brasil, a ausência de informação sobre a doença e os aspectos sociais podem aumentar o número de indivíduos com doença falciforme no município ou áreas vizinhas.

This research performed the screening of S and C hemoglobins and a social profile study of four Quilombola communities. The study was performed at the communities of Malhadinha, Córrego Fundo, Curralinho do Pontal and Manoel João Quilombola, located in the Brejinho de Nazaré municipality, Tocantins state, Brazil. The hemoglobin profile was obtained by screening on 167 blood samples using the electrophoresis in alkaline pH (pH 8.6) test. Chemically altered hemoglobin was subject to electrophoresis in acid pH (pH 6.2) to confirm the diagnosis. We identified 11 samples (6.6%) with hemoglobin changes, eight (4.8%) had sickle cell trait and three were C hemoglobin (1.8%). Social data was obtained by interviews conducted with members of 48families, raising information such as family income, age, gender and occupational activity. Our results showed that these communities are formed predominantly by adults and elderly people with monthly revenue higher than the minimum wage, accounting for 50% of the interviewed families. Although the percentage of hemoglobin S and C found in all the communities are within the values observed within the various regions of Brazil, the absence of information about the disease and social aspects may increase the number of people with sickle cell disease in the municipality and surrounding areas.

Neste trabalho foi realizada a triagem das hemoglobinas S e C e o estudo do perfil social de quatro comunidades quilombolas. O estudo foi desenvolvido nas comunidades quilombolas de Malhadinha, Córrego Fundo, Curralinho do Pontal e Manoel João, município de Brejinho de Nazaré (TO). O perfil das hemoglobinas foi obtido com a triagem de 167 amostras de sangue, utilizando o teste de eletroforese em pH alcalino (pH 8,6). Para confirmar o diagnóstico, as amostras alteradas foram submetidas à eletroforese em pH ácido (pH 6,2). Foram identificadas 11 amostras (6,6%) com hemoglobinas variantes: oito (4,8%) com traço falciforme e três com hemoglobina C (1,8%). Os dados sociais foram obtidos através de entrevista realizada com representantes de 48 famílias, levantando informações sobre a renda familiar, idade, sexo e atividade ocupacional de cada indivíduo. O estudo mostrou que essas comunidades são formadas predominantemente por adultos e idosos, com renda mensal maior que um salário mínimo, para 50% das famílias entrevistadas. Apesar do percentual das hemoglobinas S e C encontradas nas comunidades estarem dentro do observado para várias regiões do Brasil, a ausência de informação sobre a doença e os aspectos sociais podem aumentar o número de indivíduos com doença falciforme no município ou áreas vizinhas.

This research performed the screening of S and C hemoglobins and a social profile study of four Quilombola communities. The study was performed at the communities of Malhadinha, Córrego Fundo, Curralinho do Pontal and Manoel João Quilombola, located in the Brejinho de Nazaré municipality, Tocantins state, Brazil. The hemoglobin profile was obtained by screening on 167 blood samples using the electrophoresis in alkaline pH (pH 8.6) test. Chemically altered hemoglobin was subject to electrophoresis in acid pH (pH 6.2) to confirm the diagnosis. We identified 11 samples (6.6%) with hemoglobin changes, eight (4.8%) had sickle cell trait and three were C hemoglobin (1.8%). Social data was obtained by interviews conducted with members of 48families, raising information such as family income, age, gender and occupational activity. Our results showed that these communities are formed predominantly by adults and elderly people with monthly revenue higher than the minimum wage, accounting for 50% of the interviewed families. Although the percentage of hemoglobin S and C found in all the communities are within the values observed within the various regions of Brazil, the absence of information about the disease and social aspects may increase the number of people with sickle cell disease in the municipality and surrounding areas.

INTRODUÇÃO: Há uma grande carência na literatura de estudos aprofundando o conhecimento em relação às ações do lactato e algumas limitações nas investigações com indivíduos saudáveis ou portadores de algum transtorno metabólico. OBJETIVO: Verificar se é possível avaliar a capacidade aeróbia de ratos obesos induzidos por dieta através do teste de duplos esforços. MÉTODOS: Quatorze ratos machos Wistar foram divididos em dois grupos: Controle (Con) e Obeso (Obe). Os animais controles foram alimentados com ração-padrão e água fornecida ad libitum. O grupo de ratos obesos foi alimentado com ração padrão e dieta hiperlipídica. Após 12 semanas do início da dieta foi realizado o teste de tolerância à insulina, de máxima fase estável de lactato (MFEL) e o teste de duplos esforços. RESULTADOS: A dieta foi eficiente para induzir a obesidade nos animais. Os animais obesos apresentaram redução na sensibilidade à insulina de aproximadamente 19% (Con = 2,156 ± 0,1187 UA versus Obe = 1,742 ± 0,1551 UA). No teste de máxima fase estável, a concentração de lactato e velocidade correspondente ao limiar anaeróbio foram 3,780 ± 0,09 mmol/L e 18 m.min-1, para ambos os grupos. A velocidade de limiar estimada pelo teste de duplos esforços foi de 15,59 ± 0,653 m.min-1 para os animais controles e 14,02 ± 0,565 m.min-1 para os animais obesos. O teste de duplos esforços subestimou a capacidade aeróbia dos animais controles em 13% e obesos em 8,7%, contudo, apresentou correlação significativa com a MFEL (r = 0,88; P < 0,0075 Con / r = 0,92; P < 0,0031 Obe). CONCLUSÃO: O teste de duplos esforços pode ser uma alternativa interessante para avaliar a capacidade aeróbia tanto de animais sedentários saudáveis como de animais obesos.

INTRODUCTION: The literature lacks studies about lactate actions and some limitations in studies involving healthy individuals or patients with some metabolic disorder. OBJECTIVES: This study aimed to evaluate the protocol of double effort test for obese-induced rats. METHODS: Fourteen male Wistar rats were divided into two groups: Control (Con) and Obese (Obe). The control group was fed with standard chow and water ad libitum. The obese group was fed with standard chow, water ad libitum and hyperlipidic diet. Twelve weeks after the beginning of the hyperlipidic diet, insulin tolerance test, Maximal Lactate Steady State (MLSS) test and the double efforts test were performed. RESULTS: The diet was effective to promote obesity. The obese group decreased insulin sensitivity in approximately 19% (Con = 2.156 ± 0.1187 AU vs Obe = 1.742 ± 0.1551 AU). The lactate concentration and velocity of anaerobic threshold at MLSS test were 3.780 ± 0.09 mmol/L e 18 m.min-1 in both groups. The velocity of anaerobic threshold estimated by double efforts test was 15.59±0.653 m.min-1 in Con group control animals and 16.42±0.672 m.min-1 in Obe group. The double effort test underestimated around 13% and 8.7% the aerobic capacity in control and obese groups respectively, however, presented significant correlation with MLSS (r = 0,88; P < 0,0075 controls / r = 0,92; P < 0,0031 obese). CONCLUSION: So, the double effort test can be an interesting alternative to evaluate the aerobic capacity for both healthy sedentary and obese animals.

Este trabalho teve por objetivo avaliar o efeito da cera de carnaúba (Primax Wax) e filme plástico de polietileno de baixa densidade "X-tend" sobre a vida útil pós-colheita de mamão 'Formosa' (Tainung 1) produzido em Baraúna - RN. O experimento foi conduzido no laboratório de Pós-colheita da Universidade Federal Rural do Semi-Árido (UFERSA). Utilizou-se um esquema fatorial 3 x 6 com 3 repetições de três frutos por parcela, constituindo na combinação de 3 tipos de atmosferas (cera, filme de polietileno e controle) com 6 tempos de armazenamento (0; 7; 14; 21; 28; 35). Os frutos dos distintos tratamentos foram armazenados em câmaras frias a 10 ± 2 ºC e UR 90 ± 5%, analisados em intervalos de sete dias. As características avaliadas foram: aparência externa, perda de massa, firmeza da polpa, sólidos solúveis, acidez titulável e vitamina C. Constatou-se ser o filme plástico o mais eficiente em manter a qualidade externa e reduzir a perda de massa dos frutos. A cera de carnaúba, na concentração utilizada, mostrou eficiência relativa na conservação de mamão quando comparada ao filme plástico. A vida útil pós-colheita dos frutos mantidos sob atmosfera modificada foi de 35 dias e do controle apenas 28 dias.

The aim of this work was to evaluate the effect of carnauba wax (Primax Wax) and low-density polyethylene film "X-tend" on shelf life of 'Formosa' papaya fruits produced in Baraúna, RN. The experiment was carried out in the Postharvest Laboratory of the Universidade Federal Rural do Semi-Árido (UFERSA). A 3 x 6 factorial scheme with three replications of three fruits per plot in a completely randomized design was used. The treatments consisted of the combination of three types of atmosphere (wax, polyethylene film and control) with six storage times (0; 7; 14; 21; 28 and 35). The fruits of these treatments were stored in cold chamber at 10 ± 2 ºC and RH 90 ± 5%, and analyzed in 7 days intervals. The evaluated traits were: external appearance, weight loss, pulp firmness, soluble solids, titratable acidity and C vitamin. It was observed that polyethylene film was the most efficient coverage to keep the external quality and to reduce the weight loss of the papaya fruits. The carnauba wax in the used concentration shows good efficiency in papaya fruits conservation as compared with that polyethylene film. The shelf life of papaya fruits kept under modified atmosphere was of 35 days and for those fruits kept at ambient condition was of 28 days.

Os estafilococos coagulase-negativos (ECN) fazem parte da microbiota normal da pele e, apesar de terem sido considerados saprófitas por muito tempo, o seu significado clínico como agente etiológico tem aumentado com o passar dos anos. Neste estudo, foram obtidos 72 isolados de ECN a partir de amostras do conduto auditivo de cães, de mastite bovina e de infecções humanas. Staphylococcus xylosus foi o microrganismo mais isolado, nas amostras animais, e S. cohnii subsp. cohnii em humanos. Os isolados foram avaliados de modo a traçar o perfil fenotípico de sua resistência aos antimicrobianos mais indicados no tratamento de infecções estafilocócicas. Foi detectado um elevado nível de resistência à penicilina e ampicilina. A gentamicina, a vancomicina e a associação ampicilina+sulbactam foram eficientes frente aos isolados testados. A resistência à oxacilina foi avaliada por meio dos testes de difusão em disco modificada, ágar screen, microdiluição em caldo e diluição em ágar para constatar, se à semelhança do que ocorre com os estafilococos coagulase-positivo, esta pode ser mediada pelo gene mecA e apresentada de forma heterogênea. A presença do gene mecA foi determinada pelo método da Reação em Cadeia de Polimerase (PCR), sendo 5,6% dos isolados mecA positivos.

Coagulase-negative staphylococci (SCN) make part of the normal microbiota skin and although they have been considered saprophytics for years, nowadays their clinical significance as an etiologic agent has increased. In this study, 72 SCN isolates obtained from external ear canals of dogs, bovine mastitis and human nosocomial infections were evaluated. Staphylococcus xylosus was the most prevalent microorganism in animal samples and S. cohnii subsp. cohnii in human samples. SCN isolates were evaluated in order to establish a phenotypical resistance pattern towards the most indicated antibiotics for staphyloccocal infections. A high level of resistance to penicillin and ampicillin was detected. The most efficient antibiotics evaluated were gentamicin, vancomicin and the association between ampicillin and sulbactam. To certify the heterogeneous resistance pattern, oxacillin resistance was phenotypically detected by a modified-disc-diffusion test, agar screen, broth micro-dilution and agar dilution. The presence of the mecA gene was detected in 5.6% of the SCN isolates by Polimerase Chain Reaction (PCR).

Espécies de Staphylococcus spp resistentes a antimicrobianos representam um problema cosmopolita, sendo o controle de sua disseminação um importante desafio. O perfil de resistência a antimicrobianos de isolados de Staphylococcus aureus e Staphylococcus intermedius em amostras clínicas humanas e animais foi avaliado através do método de difusão em disco, no qual foi possível detectar um elevado nível de resistência à ampicilina e à penicilina. A avaliação da resistência à oxacilina, devido à heterogeneidade de resposta do gênero estudado, foi desenvolvida também através das seguintes técnicas: difusão em ágar modificado, ágar screen e microdiluição em caldo, e posteriormente correlacionada com a detecção do gene mecA, pela técnica de PCR, nas amostras consideradas resistentes em pelo menos um dos testes utilizados. A correlação entre os resultados obtidos nos testes fenotípicos com a presença do gene de resistência, considerado um método de referência, foi utilizada para validar a sensibilidade destes. De um total de 80 amostras avaliadas, 28 apresentaram resistência à oxacilina, sendo possível detectar a presença do gene de resistência mecA em 12 destas amostras. Os testes de suscetibilidade à oxacilina apresentaram sensibilidade superior a 50,0%, sendo a difusão em disco simples e o ágar screen considerados mais sensíveis, e a difusão em disco modificada, o de menor sensibilidade.

Antimicrobial resistant Staphylococcus species represent an important cosmopolitan problem, and its spreading control is a significative challenge. Resistance pattern of Staphylococcus aureus and Staphylococcus intermedius species isolated from animals and humans clinical samples to different antibiotics was evaluated through disk diffusion method, where ampicillin and penicillin presented the highest level of resistance. The evaluation of the resistance to oxacillin, due to the heterogeneity of the response of the studied genus was carried out through the following tests: modified agar diffusion, agar screen and microdilution, and further correlation with the detection of mecA gene in samples that showed resistance in at least one of the susceptibility tests used. The correlation between the results obtained from phenotypic methods and the detection of resistance gene, considered as a reference method, was used in order to validate its sensitivity. Eighty clinical staphylococcal isolates (29 human and 51 animal isolates) were evaluated, 28 were oxacillin-resistant, mecA gene being detected in 12 samples. Susceptibility assessment tests to oxacillin presented above 50% of specificity, disk diffusion and agar screen being the most sensitive one, while modified disk diffusion presented the lowest sensibility rate. Ampicillin and penicillin presented the highest level of resistance.